FSTest Chloramphenicol Rapid Test Kit

Product Overview

The FSTest Chloramphenicol Rapid Test Kit is an immunochromatographic assay designed for the qualitative detection of chloramphenicol residues in seafood, livestock/poultry meat, eggs, and honey samples. Utilizing a sandwich lateral flow immunochromatography principle with pre-coated recombinant antigens, the test cassette provides rapid, visual, and accurate results directly observed by the naked eye without requiring complex laboratory instrumentation.

Key Benefits

· Ultra-High Sensitivity: Achieves a low Limit of Detection (LOD) of 0.1 µg/kg across diverse food matrices.

· Multi-Matrix Compatibility: Optimized for seafood (fish, shrimp), meat (chicken, pork, beef), poultry eggs (chicken, duck), and honey.

· High Specificity: Tested negative with 100 µg/L of mefenthiazole, florfenicol, sulfonamides, quinolones, and other potential interfering substances.

· Rapid On-Site Screening: Delivers clear visual results directly on the cassette window within 5–8 minutes.

Technical Specifications

Applicable Sample Types

· Seafood Products: Fish and shrimp tissue samples

· Meat Tissues: Chicken, pork, and beef muscle tissues

· Poultry Products: Chicken eggs and duck eggs (yolk and egg white)

· Bee Products: Honey samples

Test Procedure

Step 1: Seafood / Meat Pretreatment
Weigh 3.0 ± 0.1g homogenized sample into a 15mL centrifuge tube. Add 5mL Chloramphenicol Reagent A, shake up/down for 3 min, centrifuge at 4000 rpm for 3 min. Evaporate 3mL supernatant at 65°C to dryness. Add 0.5mL Chloramphenicol Reagent B and 0.5mL diluting solution, vortex for 1 min, and let stand to separate (or centrifuge at 4000 rpm for 3 min). Use lower layer for testing.

Step 2: Egg Pretreatment
Weigh 3.0 ± 0.1g smashed egg sample (white and yolk) into a 15mL centrifuge tube. Add 5mL Chloramphenicol Reagent A and 1 bag Chloramphenicol Reagent C, shake up/down for 3 min, centrifuge at 4000 rpm for 3 min. Evaporate 3mL supernatant at 65°C to dryness. Add 0.5mL Chloramphenicol Reagent B and 0.3mL diluting solution, vortex for 1 min, and let stand to separate. Use lower layer for testing.

Step 3: Honey Pretreatment
Weigh 3.0 ± 0.1g honey sample into a 15mL tube, add 3mL purified water, and mix for 2 min (warm at 60–80°C for 1–2 min if needed). Add 5mL Chloramphenicol Reagent A, mix for 3 min, centrifuge at 4000 rpm for 3 min. Evaporate 3mL supernatant at 65°C to dryness. Add 0.5mL diluting solution, vortex for 1 min, and test directly.

Step 4: Assay Execution & Reading
Ensure cassette is at 20–40°C. Pipette 100µL sample solution into a microwell, mix 3–5 times, and stand for 3 min. Transfer all solution into the sample well of the test cassette and interpret results at 5–8 minutes (invalid after 8 minutes).

Performance & Validation

The FSTest Chloramphenicol Rapid Test Kit provides qualitative preliminary screening for chloramphenicol residues at a Limit of Detection (LOD) of 0.1 µg/kg.

Result Interpretation:

· Negative (-): Both C (Control) line and T (Test) line appear.

· Positive (+): C line appears; T line does not appear or is noticeably lighter (T-line intensity is inversely proportional to chloramphenicol concentration).

· Invalid: C line fails to appear, regardless of T line presence.

Compatible Instruments

Kit Components Included: 10 Test cassettes, 10 Microwells, Diluting solution (7mL) x 1, Chloramphenicol Reagent A (60mL) x 1, Chloramphenicol Reagent B (15mL) x 1, Chloramphenicol Reagent C x 1, 10 Centrifuge tubes (15mL), 10 Centrifuge tubes (10mL), and User manual.

Required Auxiliary Equipment (Not Provided): Homogenizer, vortexer, micropipettes, centrifuge (capable of 4000 rpm), electronic scale, timer, nitrogen (air) dryer or hair dryer (65°C).