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Instrument-Free & Fast: Detecting Organophosphate and Carbamate Residues in 15 Minutes
In the fresh produce industry, speed to market is everything. However, toxic pesticide residue non-compliance poses severe regulatory and financial risks to food producers, distributors, and retailers. Traditional laboratory chromatography (GC-MS / LC-MS) provides precise quantitative data but takes days to generate reports—time that fresh fruits and vegetables simply do not have.
The FSTest Pesticide Combo Card (Cat NO.: 23101 / 23111) bridges this gap. Based on the standard Cholinesterase (ChE) enzyme inhibition method, it delivers clear, qualitative Pass/Fail results in 10 to 15 minutes directly at the farm gate, wholesale market, or receiving dock—completely instrument-free.
1. What is the FSTest Pesticide Combo Card?
The FSTest Pesticide Combo Card is a portable, ready-to-use enzyme inhibition test card designed for high-throughput field screening of Organophosphate and Carbamate pesticide residues in fresh vegetables, fruits, tea, and botanical produce.
The card features a dual-tablet reaction system: a white tablet containing active cholinesterase enzyme and a red tablet containing a chromogenic substrate (indophenol acetate). When clean produce extract is applied, the enzyme hydrolyzes the substrate, turning the white tablet sky blue (Negative/Safe). If pesticide residues exceed safe limits, enzyme activity is inhibited, leaving the tablet white or light blue (Positive/Residue Present).
2. Proven Precision: Independent Validation Data
In addition to full compliance with national rapid testing standards (GB/T 5009.199), the FSTest Pesticide Combo Card has been thoroughly evaluated by independent institutions.
Evaluation data from Chiang Mai University confirmed excellent Limits of Detection (LOD) across representative vegetable matrices:
Target Pesticide | Chemical Group | Vegetable Matrix | Limit of Detection (LOD) |
Dichlorvos | Organophosphate | Yard long bean, Bok choy, Cabbage | 0.01 ppm (mg/kg) |
Methomyl | Carbamate | Yard long bean, Collard green, Bok choy | 0.01 ppm (mg/kg) |
Carbofuran | Carbamate | Bok choy, Napa cabbage, Cabbage | 0.05 ppm (mg/kg) |
Carbaryl | Carbamate | Various fresh greens | 0.50 ppm (mg/kg) |
3. Key Advantages for Modern Quality Control
- ⏱️ Rapid 10–15 Minute Turnaround:
- 🚫 Zero Equipment Required:
- 💰 90%+ Lower Cost:
- 🎯 Dual Target Screening:
- 👁️ Visual Color Contrast:
4. Simple 4-Step Testing Procedure
Streamlining on-site produce testing requires four simple steps:
- Extract:
Cut 5g of sample, mix with 10mL of pure water, shake 50+ times, and let stand for 2 minutes.
- Inoculate:
Pipette 2 drops (~80µL) of supernatant onto the white enzyme pad. Pre-react at 37°C (or body heat) for 10 minutes.
- Fold & React:
Pinch the card to fold the red substrate pad onto the white enzyme pad and hold for 3 minutes.
- Interpret Result:
Compare color change:
- 🔵 Sky Blue = Negative (Safe / No Residue)
- ⚪ White / Light Blue = Positive (Pesticide Residue Present)
5. Broad Application Across the Agricultural Supply Chain
- 🥬 Leafy Greens & Fresh Produce:
- 🍎 Fresh Fruit Distribution:
- 🍵 Tea & Medicinal Herbs:
- 🏬 Wholesale Markets & Supermarkets:
Request Free Evaluation Samples Today!
Enhance your agricultural food safety program with certified on-site rapid testing. We provide complimentary evaluation sample kits for agricultural inspectors, supermarket QC teams, and certified testing laboratories.
- 📩 Email Us: sales02@fstestcorp.com
- 🌐 Learn More:FSTest Pesticide Combo Card Product Page
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