FSTest Nitrofurantoin Metabolite (AHD) Rapid Test Kit

Product Overview

The FSTest Nitrofurantoin Metabolite (AHD) Rapid Test Kit is an immunochromatographic assay designed for the qualitative screening of 1-aminohydantoin hydrochloride (AHD), the primary metabolite of nitrofurantoin, in aquatic tissue, livestock/poultry meat, and egg samples. Utilizing a sandwich lateral flow immunochromatography principle with pre-coated recombinant antigens, the test cassette provides rapid, visual, and accurate results directly observed by the naked eye without requiring complex laboratory instrumentation.

Key Benefits

· Unified 4-in-1 Pretreatment: Shares a standardized sample extraction workflow with nitrofuran metabolite kits for SEM, AOZ, and AMOZ, enabling efficient batch multi-derivative processing.

· High Sensitivity: Achieves an ultra-low Limit of Detection (LOD) of 0.5 µg/kg across diverse food matrices.

· High Specificity: Excellent specificity with no cross-interference; tested negative with 100 µg/L of chloramphenicol, malachite green, sulfonamides, and other non-target compounds.

· Rapid & Visual Reading: Clear signal interpretation provided directly on the cassette test window within 5–8 minutes.

Technical Specifications

Applicable Sample Types

· Aquatic Products: Fish, shrimp, and shellfish tissues

· Meat Tissues: Pork and chicken muscle tissues

· Poultry Products: Whole egg (yolk and egg white)

Test Procedure

Step 1: Sample Weighing
Weigh 2.0 ± 0.1g homogenized tissue or 3.0 ± 0.1g homogenized egg into a 15mL centrifuge tube.

Step 2: Derivatization & Extraction
Add 5mL Furan Reagent A and 0.3mL Furan Reagent B, vortex for 1 min, and incubate in an 80°C water bath for 15 min. Add 1mL Furan Reagent C, 0.6mL Furan Reagent D, and 5mL Furan Reagent E. Vortex for 1 min and centrifuge at 4000 rpm for 5 min.

Step 3: Evaporation & Reconstitution
Transfer 3mL supernatant (or all if <3mL for eggs) into a 10mL centrifuge tube and evaporate to dryness at 65°C with hot air or nitrogen. Add 0.8mL Furan Reagent F and 0.5mL diluting solution, vortex for 30s, and centrifuge at 4000 rpm for 3 min (or let stand to separate). Remove the upper layer liquid; the remaining lower liquid is ready for testing.

Step 4: Assay Execution & Reading
Ensure cassette is at 20–40°C. Pipette 100µL sample solution into a microwell, mix 3–5 times, and stand for 3 min. Transfer all solution into the sample well of the test cassette and interpret results at 5–8 minutes (invalid after 8 minutes).

Performance & Validation

The FSTest Nitrofurantoin Metabolite (AHD) Rapid Test Kit provides qualitative preliminary screening for AHD contamination at a Limit of Detection (LOD) of 0.5 µg/kg.

Result Interpretation:

· Negative (-): Both C (Control) line and T (Test) line appear.

· Positive (+): C line appears; T line does not appear or is noticeably lighter (T-line intensity is inversely proportional to AHD concentration).

· Invalid: C line fails to appear, regardless of T line presence.

Compatible Instruments

Kit Components Included: 10 Test cassettes, 10 Microwells, Diluting solution (6mL) x 1, Furan Reagent A (60mL) x 1, Furan Reagent B (4mL) x 1, Furan Reagent C (15mL) x 1, Furan Reagent D (9mL) x 1, Furan Reagent E (50mL) x 1, Furan Reagent F (10mL) x 1, 10 Centrifuge tubes (15mL), 10 Centrifuge tubes (10mL), and User manual.

Required Auxiliary Equipment (Not Provided): Homogenizer or grinder, water bath (80°C), vortexer, micropipettes, centrifuge (capable of 4000 rpm), electronic scale, timer, nitrogen (air) dryer or hair dryer (65°C).