FSTest Nitrofurazone Metabolite (SEM) Rapid Test Kit

Product Overview

The FSTest Nitrofurazone Metabolite (SEM) Rapid Test Kit is an immunochromatographic assay designed for the qualitative screening of semicarbazide (SEM), the primary metabolite of nitrofurazone, in animal tissues and poultry egg samples. Utilizing a sandwich lateral flow immunochromatography principle with pre-coated recombinant antigens, the test cassette provides rapid, visual, and accurate results directly observed by the naked eye without requiring complex laboratory instrumentation.

Key Benefits

· Unified Sample Pretreatment: Compatible with FSTest rapid test kits for furazolidone (AOZ), furaltadone (AMOZ), and nitrofurantoin (AHD) metabolites, enabling a 4-in-1 multi-nitrofuran derivative extraction process.

· High Sensitivity: Achieves a low Limit of Detection (LOD) of 0.5 µg/kg across diverse food matrices.

· Broad Matrix Compatibility: Fully validated for aquatic tissues (fish, shrimp, shellfish), livestock/poultry meat (pork, chicken), and eggs.

· Rapid & Visual Reading: Clear signal interpretation provided within 5–8 minutes post-incubation.

Technical Specifications

Applicable Sample Types

· Aquatic Products: Fish, shrimp, and shellfish tissues

· Meat Tissues: Pork and chicken muscle tissues

· Poultry Products: Whole egg (egg yolk and egg white)

Test Procedure

Step 1: Sample Weighing
Weigh 2.0 ± 0.1g homogenized tissue or 3.0 ± 0.1g homogenized egg into a 15mL centrifuge tube.

Step 2: Derivatization & Extraction
Add 5mL Furan Reagent A and 0.3mL Furan Reagent B, vortex for 1 min, and incubate in an 80°C water bath for 15 min. Add 1mL Furan Reagent C, 0.6mL Furan Reagent D, and 5mL Furan Reagent E. Vortex for 1 min and centrifuge at 4000 rpm for 5 min.

Step 3: Evaporation & Reconstitution
Transfer 3mL supernatant (or all if <3mL for eggs) into a 10mL centrifuge tube and evaporate to dryness at 65°C with hot air or nitrogen. Add 0.8mL Furan Reagent F and 0.5mL diluting solution, vortex for 30s, and centrifuge at 4000 rpm for 3 min (or let stand to separate). Remove the upper layer liquid; the remaining lower liquid is ready for testing.

Step 4: Assay Execution & Reading
Ensure cassette is at 20–40°C. Pipette 100µL sample solution into a microwell, mix 3–5 times, and stand for 3 min. Transfer all solution into the sample well of the test cassette and interpret results at 5–8 minutes (invalid after 8 minutes).

Performance & Validation

The FSTest Nitrofurazone Metabolite (SEM) Rapid Test Kit provides qualitative preliminary screening for SEM contamination at a Limit of Detection (LOD) of 0.5 µg/kg.

Result Interpretation:

· Negative (-): Both C (Control) line and T (Test) line appear.

· Positive (+): C line appears; T line does not appear or is noticeably lighter (T-line intensity is inversely proportional to SEM concentration).

· Invalid: C line fails to appear, regardless of T line presence.

Compatible Instruments

Kit Components Included: 10 Test cassettes, 10 Microwells, Diluting solution (6mL) x 1, Furan Reagent A (60mL) x 1, Furan Reagent B (4mL) x 1, Furan Reagent C (15mL) x 1, Furan Reagent D (9mL) x 1, Furan Reagent E (50mL) x 1, Furan Reagent F (10mL) x 1, 10 Centrifuge tubes (15mL), 10 Centrifuge tubes (10mL), and User manual.

Required Auxiliary Equipment (Not Provided): Homogenizer, water bath (80°C), vortexer, micropipettes, centrifuge (capable of 4000 rpm), electronic scale, timer, nitrogen (air) dryer or hair dryer (65°C).